Isolation of bnAbs

Methods for HIV bnAb isolation. (A) mAb isolation by phage library from plasma cells and subsequent phage display to enrich for antigen‐specific clones; (B) mAb isolation by immortalization of total B cells. Propagated cells are then serially diluted and Abs secreted in the supernatant tested for antigen specificity; (C) mAb isolation by single B‐cell culture without immortalization, Abs secreted in the supernatant tested for antigen specificity and Ab sequences obtained; (D) mAb isolation by antigen‐specific single B‐cell FACS. Ab sequences are amplified from each well and tested for antigen specificity.

Adapted from Immunological Reviews
Volume 275, Issue 1, pages 11-20, 30 JAN 2017 DOI: 10.1111/imr.12484
http://onlinelibrary.wiley.com/doi/10.1111/imr.12484/full#imr12484-fig-0001