Methods for HIV bnAb isolation. (A) mAb isolation by phage library from plasma cells and subsequent phage display to enrich for antigen‐specific clones; (B) mAb isolation by immortalization of total B cells. Propagated cells are then serially diluted and Abs secreted in the supernatant tested for antigen specificity; (C) mAb isolation by single B‐cell culture without immortalization, Abs secreted in the supernatant tested for antigen specificity and Ab sequences obtained; (D) mAb isolation by antigen‐specific single B‐cell FACS. Ab sequences are amplified from each well and tested for antigen specificity.
