Reverse Vaccinology

Reverse vaccinology is a new paradigm in challenging vaccine development, which is enabled by many new technologies in immunology, structural biology, and chemistry. This novel approach can be summarized as follows:

1. Identification of 'elite responders' through analysis of chronically infected patient sera

2. Isolation of broadly neutralizing antibodies (bnAbs) from chronically infected patients

3. Determination of the nature of the glycopeptidic epitope bound by the bnAb, known as epitope mapping

4. Determination of the precise molecular interactions between the bnAb and the epitope (X-Ray or cryo-EM)

5. Design and chemical synthesis of the glycopeptidic epitope mimic - Chemitope Glycopeptide, LLC

6. Multimerization of the epitope mimic and formulation with non-specific immunostimulants

7. Immunization

Mini V3 Adapted from pdb file 3TYG; Pejchal, R.Doores, K.J.Walker, L.M.Khayat, R.Huang, P.S.Burton, D.R.Wilson, I.A. A potent and broad neutralizing antibody recognizes and penetrates the HIV glycan shield. (2011) Science 334: 1097-1103.