Boutique chemical manufacturing of homogeneous glycopeptides.

About Us

Chemitope Glycopeptide, LLC, or Chemitope, is a boutique biotech company that aims to produce a new generation of preventative vaccines. Chemitope was founded by Dr. Baptiste Aussedat and Dr. William Walkowicz, two PhD scientists at Memorial Sloan Kettering Cancer Center in New York City, to meet the current and future demand for homogeneous glycopeptides in the ongoing effort to develop an HIV vaccine. 

Our Vision

For decades, the dense layer of carbohydrates present on viral glycoproteins, known as the 'glycan shield', was viewed by the scientific community as an elaborate protection mechanism to evade the immune system. However, reverse vaccinology, which is emerging as the new paradigm in challenging vaccine development, has made that 'shield' one of its primary targets. We believe that chemically defined homogeneous glycopeptides are essential for the induction of a broadly neutralizing antibody response against insidious viruses like HIV, Hepatitis C, and Influenza.


We specialize in the design and synthesis of complex homogeneous glycopeptides, which can accurately mimic a domain or a motif of a viral glycoprotein. Our highly efficient chemical synthesis process allows for atomic-level control of the targeted glycopeptidic construct, and the incorporation of various post-translational modifications and unnatural amino acids.



Experts in Glycopeptide Engineering

The team at Chemitope Glycopeptide has extensive experience in the chemical synthesis of challenging glycopeptides and full-length glycoproteins. Our flexible methods allow for the incorporation of homogeneous natural and unnatural N- and O-glycosylation motifs along with unnatural amino acids and post-translational modifications, including, sulfation, phosphorylation, and methylation. 


Our technology to produce synthetic glycopeptides relies on the paradigm of convergent N-linked glycopeptide assembly depicted in the figure below and exemplified by the chemical synthesis of the HIV broadly neutralizing V3 epitope mimic. This technology offers us the possibility to access glycopeptidic structure regardless of the glycosylation sites' relative position in the sequence, the nature of the glycan of interest (complex-type or high-mannose) and the nature of the appended entities (adjuvants, T-helper peptide, functional handle) required for the full potentiation of these immunogens.

Immunogen Design

In the case of the HIV, shown below, a single glycoprotein, Env, coats the entire surface of the virion. Over the last decade, several sites of vulnerability have been identified at the surface of Env, opening the path towards a prophylactic HIV vaccine. Among the sites known to induce a broadly neutralizing antibody (bnAb) response, the most promising are glycopeptidic epitopes (V1V2 region, V3 loop) comprising both peptide and glycan moieties. To build an effective immune response against these key epitopes, immunization should be carried out with glycopeptides that share these characteristic features. Thus, generation of an accurate glycopeptide epitope mimic involves the conceptual excision of the motif of interest from the native glycoprotein, with the aid of structural data from X-ray, cryo-electron microscopy, or nuclear magnetic resonance spectroscopy. From there, the Chemitope team designs and synthesizes a conformationally stable glycopeptide, which is subsequently probed for antigenicity and iteratively improved by substituting sub-optimal residues and modulating the nature of the glycosylation motifs.


Once the targeted sequence is synthesized and successfully validated through antigenicity studies, the epitope mimic can be:

  • Selectively functionalized to improve immunogenicity (adjuvants, T-helper epitope, multimerization) and formulated for immunization

  • Used for structural studies with X-ray crystallography, cryo-EM, or NMR spectroscopy

  • Used for isolation of unknown bnAbs from infected patient sera, as shown in Science Translational Medicine.

Recent publications from the Chemitope Team




Our specialty is the synthesis of challenging glycopeptides. We can produce micrograms to hundreds of milligrams of homogeneous glycopeptide, which can be functionalized in almost limitless ways. For examples, see our publications in the Journal of the American Chemical Society and Science Translational Medicine (shown at right).  We will work with your team to design candidate constructs to suit your needs. Contact us for a quote.

Functionalized Glycans

Our library of synthetic and semisynthetic glycans can be purchased for use in your custom application.

  • Customized linker (PEGx, succinate, etc)

  • Functional handle (azide, alkyne, thiol, amine, etc)

  • Tag (FITC, cascade blue, etc)

Contact us for a quote!


The Team

​Baptiste Aussedat, PhD, CEO & Co-founder: Baptiste received his training at the University Pierre and Marie Curie in the Laboratory of Biomolecules under the direction of Dr. Gerard Chassaing, where he worked on the synthesis and biological evaluation of pseudo-peptides as new drug delivery systems. In 2008, he joined the laboratory of Prof. Samuel J. Danishefsky at the Memorial Sloan Kettering Cancer Center (MSKCC), where he completed the first synthesis of a purely synthetic complex glycoprotein: the human Follicle-Stimulating Hormone (hFSH). Since 2012, Baptiste has led a team from the Danishefsky group that aims to design and synthesize glycopeptide immunogens for the Duke CHAVI-ID consortium. In 2016, he was appointed principal investigator at MSKCC. Baptiste has over 14 years of experience in peptide chemistry and his work has dramatically improved the efficiency of the key transformations in glycopeptide synthesis, which is the driving force for the creation of Chemitope.

William Walkowicz, PhD, CSO & Co-founder: Bill received his training at the Gerstner Sloan Kettering Graduate School of Biomedical Sciences in the laboratory of Prof. David Y. Gin, where he focused on the design and chemical synthesis of complex immunostimulants. His studies on these large, complex, and fragile molecules resulted in the discovery of the most potent saponin immunoadjuvant to date, and proved an excellent training for his subsequent work in complex glycans and glycopeptides. After joining the Danishefsky group in 2014, he honed his expertise on the total synthesis of glycans as well as the purification of these highly valuable products from natural sources, one of the key technologies driving the financial tractability of Chemitope.

Made in Brooklyn

Product Inquiry

Contact us below for a quote, or general inquiry to assess feasibility and timelines.

Tel: 123-456-7890

140 58th st, Bldg A, Suite 8J

Brooklyn, NY 11220

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